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A Immunostaining of sagittal sections from a CS17 human embryo. Co-expression of NR2F2 (green) and SOX18 (blue) in the gut. scale bars 50 μm. B-B ”,and C-C ” Sections of proband liver (B) and duodenum (C) stained for CD31 (cyan), SOX18 (red arrows), NR2F2 (green arrows), and <t>DAPI</t> (purple). Orange and gold boxes denote zoomed in regions of individual vessels. Orange box represents vessel that shows cells co-expressing both NR2F2 and SOX18 in the same nucleus, whereas the gold box is a vessel with cells either expressing NR2F2 or SOX18. Scale bar = 50μm (B-C) and 10 μm (B’”-C””).
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A Immunostaining of sagittal sections from a CS17 human embryo. Co-expression of NR2F2 (green) and SOX18 (blue) in the gut. scale bars 50 μm. B-B ”,and C-C ” Sections of proband liver (B) and duodenum (C) stained for CD31 (cyan), SOX18 (red arrows), NR2F2 (green arrows), and <t>DAPI</t> (purple). Orange and gold boxes denote zoomed in regions of individual vessels. Orange box represents vessel that shows cells co-expressing both NR2F2 and SOX18 in the same nucleus, whereas the gold box is a vessel with cells either expressing NR2F2 or SOX18. Scale bar = 50μm (B-C) and 10 μm (B’”-C””).
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A Immunostaining of sagittal sections from a CS17 human embryo. Co-expression of NR2F2 (green) and SOX18 (blue) in the gut. scale bars 50 μm. B-B ”,and C-C ” Sections of proband liver (B) and duodenum (C) stained for CD31 (cyan), SOX18 (red arrows), NR2F2 (green arrows), and DAPI (purple). Orange and gold boxes denote zoomed in regions of individual vessels. Orange box represents vessel that shows cells co-expressing both NR2F2 and SOX18 in the same nucleus, whereas the gold box is a vessel with cells either expressing NR2F2 or SOX18. Scale bar = 50μm (B-C) and 10 μm (B’”-C””).

Journal: bioRxiv

Article Title: Inhibiting SOX18 with propranolol restores vascular integrity in NR2F2-driven malformations

doi: 10.1101/2025.04.25.650344

Figure Lengend Snippet: A Immunostaining of sagittal sections from a CS17 human embryo. Co-expression of NR2F2 (green) and SOX18 (blue) in the gut. scale bars 50 μm. B-B ”,and C-C ” Sections of proband liver (B) and duodenum (C) stained for CD31 (cyan), SOX18 (red arrows), NR2F2 (green arrows), and DAPI (purple). Orange and gold boxes denote zoomed in regions of individual vessels. Orange box represents vessel that shows cells co-expressing both NR2F2 and SOX18 in the same nucleus, whereas the gold box is a vessel with cells either expressing NR2F2 or SOX18. Scale bar = 50μm (B-C) and 10 μm (B’”-C””).

Article Snippet: Cells were then washed with FACS buffer (1:5), and resuspended in 300μl FACS buffer + DAPI ((Miltenyi Biotec, # 130-111-570) for live/dead cell identification.

Techniques: Immunostaining, Expressing, Staining